RNA In Situ Hybridization (F/ISH)
GlintLab offers comprehensive RNA In Situ Hybridization (ISH) services, including both fluorescence (FISH) and chromogenic (ISH) detection. With years of experience in technologies like RNAscope™, our team delivers end-to-end support for your RNA visualization needs, helping you achieve accurate and reproducible results.
Why Choose GlintLab for RNA In Situ Hybridization?
- Expertise in Probe and Assay Selection: We guide you in selecting the most appropriate probes and assays for your targets, whether standard or custom designs, ensuring compatibility with your specific research goals.
- Comprehensive Optimization: Our protocols are tailored to maximize signal-to-noise ratios and ensure reliable detection across diverse tissue types and RNA targets.
- Advanced Imaging and Analysis: We provide high-resolution imaging and cutting-edge analysis tools for quantitative and spatial RNA data.
RNA ISH Process
Probe Selection
- Assistance with standard or custom probe design for single or multiple RNA targets.
- Vendor selection support: Choose the best probes from trusted sources like ACD or Molecular Instruments, tailored to the length and type of RNA targets.
Assay Selection
- Selection of appropriate assays based on:
- Target sequence length: Short vs. long RNA targets.
- Expression levels: Channel recommendations for expected target abundance in multiplex assays.
- Assay type: RNA-only assays or RNA + protein detection for combined studies.
- Instrumentation guidance: Recommendations for manual or automated staining using platforms such as Leica Bond and Roche Ventana Discovery Ultra autostainers for high-throughput or complex multiplexing needs.
Staining and Imaging
- RNA integrity validation and high-resolution staining for all test samples.
- Imaging options include:
- Brightfield ISH for chromogenic detection.
- Fluorescent ISH (FISH) for multiplex RNA visualization or RNA-protein co-localization.
- Customizable workflows to fit your experimental design and imaging requirements.
Image Analysis
- Quantitative cell-by-cell RNA expression analysis to deliver actionable insights.
- Spatial RNA analysis capabilities to map gene expression within the tissue architecture.
- Data outputs designed for publication or further computational modeling.
Histopathology Preclinical CRO San Diego
